1	"Animal Biosafety Level 2" Animal Biosafety Level 2 Personnel Training
2	ALL PERSONNEL WORKING IN A ABLS2 AREA SHOULD PRACTICE THE FOLLOWING… Laboratory personnel should receive appropriate immunizations or tests for any agents handled or potentially present in the room (serum may be collected). Access is limited where the work is to be conducted. (You will need to have a key issued to you for entrance into EB08H.)
3	ALL PERSONNEL WORKING IN A ABLS2 AREA SHOULD PRACTICE THE FOLLOWING… Extreme precautions must be taken with contaminated sharp items. Use alternatives to sharps wherever possible. Procedures are conducted in a class 2 biological safety cabinet provided in the room, and where aerosols or splashes may occur, face shields should also be worn.
4	ARC Training Procedures You must attend a training session with the Training and Compliance Coordinator and be issued a key prior to working in EB08H. You must have read and fully understand your protocol and be aware of the potential hazards of the pathogens you are working with.
5	Entry Procedures Remove your lab coat/gown that is to be worn in the ARC main facility and hang it on the clothing rack provided outside of EB08H.
6	Entry Procedures
7	Entry Procedures Step into EB08H, the ABSL2 housing room for rodents. After entering EB08H, directly to your right you will find your personnel protective outerwear. Here is where you will put on ABSL2 required attire, gowns, hats, masks, and gloves. Personnel protective outerwear MUST be worn while working in EB08H. Proceed to animal housing area.
8	Conducting Your Procedures Always work in the class 2 biological safety cabinet. Always spray and wipe down all interior surfaces of the class 2 biological safety cabinet with Clidox, before and after working. Do not spray the top grille of the class 2 biological safety cabinet (this is where the filter is located). Always open mouse micro-isolator cages in the class 2 biological safety cabinet with aseptic micro-isolator technique (If you have not had this training, contact the Training and Compliance Coordinator @ 368-2507).
9	Conducting Your Procedures When infecting animals with approved pathogenic agents, you must place a Biohazard Cage Card on the cage. (ARC provides these specific cards for your use.)
10	Biohazard Cage Card Information Fill out all the information on the Biohazard Cage Card: PI Name: Phone Number: Protocol Number: Pathogenic agent: Dose per animal: Date infected: Husbandry by: PI or ARC (circle) Carcass disposal: Autoclave or Regular (circle) Bedding disposal: Autoclave or Regular (circle) Cage sanitation: Spray cages with Clidox or other decontamination agent (list other agents), Standard Cagewash (circle or Autoclave)
11	Conducting Your Procedures The ARC is responsible for all routine husbandry in ABSL2, EB08H. If you need to make additional cage changes, make sure to follow the appropriate guidelines, found in the ABSL2 Standard Operating Procedures. A copy of the SOP is located in the room data book for your convenience.
12	Conducting Your Procedures Infected bedding or carcasses must be incinerated at time of disposal, and need to be placed in a biohazard bag inside the biological safety cabinet. The bag should be tied loosely and placed in the red biohazard barrel marked “INFECTED BEDDING/ CARCASSES FOR INCINERATION AND DISPOSAL.” This Excludes PRION Infectious Wastes from room EBO9H. PRION Infectious Wastes are to be disposed of in EB09H.
13	Conducting Your Procedures Cages that had infected bedding or animals must be autoclaved, and need to be placed in a biohazard bag inside the biological safety cabinet. The bag should be tied loosely and placed in the red biohazard barrel marked “INFECTED CAGES TO BE AUTOCLAVED.” This Excludes PRION Infectious Wastes from room EBO9H. PRION Infectious Wastes are to be disposed of in EB09H.
14	Conducting Your Procedures Upon completion of your work in EB08H, all biohazard bags should be placed in the appropriate red biohazard barrels, which are located in the animal housing area. PLEASE MAKE SURE TO PLACE YOUR BEDDING/CARCASSES OR CAGES IN THE APPROPRIATELY MARKED BARREL. The ARC will process and dispose of the materials for you.
15	Conducting Your Procedures If you no longer need cages with NON-INFECTED animals, no special handling is required; you may take them down to the postmortem room, EB09A. Please make sure the animals have ample food and water. If you need to dispose of NON-INFECTED cages without animals, no special handling is required; you may take them down to the cage dump room , EB08C. Always place contaminated sharps in the approved biohazard container.
16	Conducting Your Procedures Report all spills and accidents that result in overt exposure to infectious materials to an ARC Husbandry Manager and the ARC office 368-3490. Always spray and wipe down all interior surfaces of the class 2 biological safety cabinet and all other work surfaces with Clidox after working.
17	Exiting Procedures When all of your work is completed, your animal cages are back on the housing rack, your infected wastes have been placed in the appropriate red biohazard barrel and the class 2 biological safety cabinet and all other work spaces have been decontaminated with Clidox, you may remove your gloves.
18	Exiting Procedures After washing your hands DO NOT TOUCH ANY SURFACES IN THE HOUSING AREA, proceed directly to the changing area. Remove your hat and mask, and discard them in the biohazard trash can provided in the changing area.
19	Exiting Procedures Remove your gown and place in the biohazard laundry bin, provided in the changing area. DO NOT EXIT THE ROOM WITH YOUR PERSONAL PROTECTIVE OUTERWEAR, these items must stay in the room to be laundered or disposed of by the ARC. All gowns will be autoclaved or laundered by the ARC.
20	Exiting Procedures After exiting EB08H, wash your hands AGAIN in the Prep Room, EB05H directly across the hall.
21	Completion Safety is everyone’s responsibility. Following procedures outlined in this document is essential to ensure the safety of all personnel Take the test by following the link. If you don’t know an answer, be sure and review the presentation. Take Test